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Apart from these, no further extracellular proteases share this particular substrate motif, suggesting that many cleavage events seen in vivo exhibiting negatively charged amino acid residues in P1′ might be due to meprin activity, or related family members.
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Many Drosha-dependent mRNA cleavage events were identified in mESCs, consistent with Microprocessor regulation of coding mRNAs through direct cleavage (Karginov et al., 2010).
It is worth bearing in mind that the substrate of Cas6 is a large pre-crRNA with many cleavage sites and that two RNA-cleavage events are required to generate one unit-length crRNA.
Intuitively, many of the observed products F ij are due to uncorrelated cleavage events B j and reverse transcription stop events R i, which produce a 'plaid' background pattern.
Is there such a thing as too many cleavage options?
Because of their loose structures, many cleavage sites in these peptides are exposed to proteolytic enzymes.
Pattern analysis of cleavage events indicated putative targeting sequences during the GV-MII transition.
Two LSU rRNA fragments appear first, followed by smaller fragments produced by additional cleavage events.
A larger fragment appeared to accumulate earlier in the course of infection than the smaller fragments, suggesting a specific and sequential cleavage, with a primary cleavage followed by secondary cleavage events.
No such cleavage events were observed in 8226 GFP cells.
Ribosome biogenesis involves a number of pre-rRNA cleavage events.
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