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Several classes within the family include multiple species orthologs, and within each ortholog, many binding proteins have been crystallized within different states (i.e., presence/absence of ligand, wild-type vs. mutant, etc).. Fig. 2a shows the neighbor-joining phylogenetic tree built from the Probalign [32, 33] multiple sequence alignment of 29 representative structures.
FREX can in principle be applied to many binding proteins to generate a consistent ligand-dependent conformational change.
All of these studies were limited to identifying a single major antennal protein, although studies in other organisms indicate that many binding proteins likely contribute to olfaction [ 25, 34].
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Next, we replaced Ca2+ with Tb3+, which can bind in place of Ca2+ in many Ca2+ binding proteins due to its similar ionic radius.
Acquisition of motility is an important step in malignant progression of tumor cells and involves dynamic changes in actin filament architecture orchestrated by many actin binding proteins.
Genetic screens indicate that many RNA binding proteins play important roles in N signaling [27] [28].
Furthermore, WRN interacts physically and functionally with many DNA binding proteins involved in DNA repair processes.
A zinc binding finger is present in a similar region of RAG-1 and the DBP, however zinc sites are present in many DNA binding proteins [17].
They might also be useful for studies aimed at understanding how the many RNA binding proteins identified in genetic screens fit into the regulation of N signaling.
Domains I and III (Fig. S2) are similar to the corresponding domains in many substrate binding proteins and the interface between domains I and III likely comprises the peptide binding pocket.
Many membrane binding proteins induce membrane deformation.
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