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In addition, to the hits we further analyzed, there are many additional markers from our data set to explore that could potentially identify unique subpopulations of NSC, neurons and glia.
Finally, we found six new associations with SNPs in ERAP1 with AS, and many additional markers by imputation which are possible candidates for the causative variant(s) in this gene.
We note that many additional markers were genotyped in samples that were previously reported, and that Cohanim samples reported here do not overlap with the original collection of Skorecki et al. (1997) and Thomas et al. (1998).
Note, however, Lander and Kruglyak (1995) strongly recommend the use of a genome-wide significance threshold, regardless of the density of the map used, to further deploy many additional markers in regions that show signs of a segregating QTL after an initial sparse map search (Van Ooijen 1999).
We were able to define the Rlm2 locus to a map interval of 5.8 cM and a physical interval of 191 predicted B. napus genes, though further dissection of the Rlm2 interval was hampered by a lack of polymorphism, as many additional markers located within the region proved to be monomorphic in our population.
The number of significant markers for each test, and the statistical power of the test are also presented in Table 2. Power was generally low, indicating that many additional markers may have been in linkage to QTL but did not reach statistical significance.
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It is noteworthy that many additional cell-membrane markers, with similar secondary structures, were also resolved (Additional file 3: Table S3) and may serve as a valuable pool of candidate markers for future studies.
Much lower density of reads/sample for those normalised using the Sequalprep kit as many more samples and additional markers included in run however coverage was far more even across samples and markers and averaged at almost 200× per sample.
Many groups have identified alternative or additional markers, among which PCA3, in order to detect early prostate cancer through screening, to distinguish potentially lethal from indolent prostate cancers, and to guide the treatment decision.
For example, many additional peaks that appeared in this study coincided with pseudo markers in large marker intervals.
While a limited number of SSR markers were obtained from genomic libraries [ 6, 22- 24], the implementation of large EST databases allowed the development of many more SSR markers [ 25, 26], and additional markers have been developed from Clementine BACs end sequencing (BES; [ 27- 29]).
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