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Like all eukaryotes (organisms whose cell nuclei are enclosed within a membrane) Drosophila's genome is divided among euchromatin, which contains many active genes, and heterochromatin, which – although it amounts to about a third of the genome – contains relatively few active genes.
They are predominantly found in many active genes and cis-regulatory elements [ 4].
Moreover, for many active genes the transcription factors contained only two of these TAFs.
TFIIB has recently been shown to cross-link with both the terminator and promoter regions of many active genes (Singh and Hampsey, 2007).
ASH1L occupies many active genes (Gregory et al., 2007), and TrxG family proteins are involved in many cell fate decisions in development and disease (Mills, 2010; Smith et al., 2011).
Importantly, Tip60, NSL and MSL coactivator HAT complexes have a genome-wide overlap at many active genes, but their specific functions might be reflected in their distinct binding profiles around the TSSs.
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To examine how many active transcribing genes and pausing genes were identified by RNAseq, we constructed a Venn diagram from ChIPseq and RNAseq data.
This view, however, may be an oversimplification, since many highly active genes are not depleted of nucleosomes [ 23].
Heterochromatinization progresses with aging and can deactivate many previously functioning active genes.
Indeed, 5hmC levels increase during neuronal differentiation and a very stable intragenic enrichment of 5hmC was observed at many active neuron-specific genes [ 66, 68– 70].
Moreover, all the three i-type genes in many T. urartu accessions were active genes, while only one or two genes were expressed in common wheat.
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