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A brief conclusion is given in Section 5, and abbreviations used in the manuscript are listed in the "Abbreviation" section.
Some structural comments and concrete suggestions for improving the manuscript are listed below.
All primers used in this manuscript are listed in [Additional file 2].
All GFP-strains used in this manuscript are listed in Additional file 16.
Significantly differently expressed proteins and common names of proteins discussed in the manuscript are listed in Additional file 2: Table S2.2.
Accession numbers for RH4 and ATCC 43617, as well as the version described in this manuscript, are listed in Table 1.
The transcripts for mex3-1 all all new progeny markers from this manuscript are listed in Supplementary file 2. Asexual S. mediterranea CIW4 strain was reared as previously described (Sánchez Alvarado et al., 2002).
The times required for each step, and the proportion of "hands-on" time for the process that was current at the time of the submission of the revised manuscript is listed in Table 1.
All GenBank accession identifiers for the sequences analyzed in this manuscript are listed in Additional file 2. Statistical sampling predicts that this represents a substantial fraction of the total transcriptome.
The following abbreviations used in the manuscript are listed here in alphabetical order: ME: (minimum evolution); ML: (maximum-likelihood); NJ: (neighbor-joining); PGN: (peptidoglycan); PGRPs: (Peptidoglycan Recognition Proteins); RACE: (Rapid amplification of cDNA ends); SLAC: (Single Likelihood Ancestor Counting).
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