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Half the subjects started with examiner A, and half with examiner B. Both examiners performed the tests in the same order on each subject, specified in the current manuscript (Test 1-5) in the section 'Tests for lumbar motor control'.
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Essential revisions: 1) Figure 2: the first experiment in the manuscript tests the effect of replacing phenylalanine residues in the TM span with leucine or alanine residues.
We submitted previously to eLife a manuscript testing the roles of REST and Polycomb repression in regulating the neuronal lineage in mouse embryonic stem cells.
2) The manuscript tests multiple hypotheses – structure the Methods and Results section so that approach and results for each hypothesis being tested can be tracked easily by the reader.
The Rothstein et al. Nature paper that is cited in the manuscript tested ceftriaxone on synaptically-evoked currents in astrocytes in hippocampus but there was not a significant effect.
In the current manuscript, we test our hypothesis using fluorescence recovery after photobleaching (FRAP) to determine the dynamics of H-RasG12V-Gal-1 complexes on the plasma membrane of live cells, and immunogold EM spatial mapping to determine recruitment of Raf to H-RasG12V-Gal-1 nanoclusters.
2) It would greatly strengthen the manuscript to test whether specific activation of the neuregulin pathway in cardiomyocytes is sufficient for this dramatic effect.
In this manuscript, they test the in vivo effect of changes in the intracellular concentration of σ on the proportion of TECs that contain σ.
In this manuscript, we test this hypothesis by developing a new method named Guided Latent Factor Discovery (GLFD) to find such factors if they exist.
In this manuscript, the tested devices possess a stand-alone crossbar configuration with an active area of 5 × 5 μm2.
In the current manuscript, we tested several predictions that develop from the FIT in order to localize damage in the functional architecture of the language system.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com