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DJB participated in writing the manuscript, marker development, analysis of data and co-ordination of the study.
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In addition to Cd45 and Cd 68 reported in the manuscript, other markers for immune cells (T cells, B cells and leukocytes) did not show significant correlation in hepatic TG contents.
In the original manuscript we chose markers that best illustrated the phenotype described in the specific part of the manuscript.
MI and PM conceived the study, performed statistical data analysis, and wrote the manuscript; MI performed serum marker analysis; OC and EN performed the PETCT clinical evaluation; MA performed the data processing; CP revised the statistical data analysis; AS, OC, and EN reviewed and revised the manuscript for important intellectual content.
In the revised manuscript, besides the vm1 specific marker egl-15::gfp, we have included analyses of two additional cell-type specific markers (hlh-8 gfp hlh-8 gfp:gfp).
F.A.G. participated in the microsatellite marker validation and manuscript editing.
C.v.d.B. participated in the microsatellite marker validation and manuscript editing.
HKB coordinated library construction and sequencing of a large proportion of the ESTs, experimental design, marker analysis and manuscript development.
We don't discuss these results in the manuscript because without a second marker to follow the vesicle, we cannot definitively know that the bleached vesicle is still in the focal plane.
MN designed the staining tests and optimized the pretreatments, selected most markers, wrote the manuscript and prepared the figures.
The transcripts for mex3-1 all all new progeny markers from this manuscript are listed in Supplementary file 2. Asexual S. mediterranea CIW4 strain was reared as previously described (Sánchez Alvarado et al., 2002).
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