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Clément, N. & Grieger, J. C. Manufacturing of recombinant adeno-associated viral vectors for clinical trials.
Lock, M. et al. Rapid, simple, and versatile manufacturing of recombinant adeno-associated viral vectors at scale.
Fed-batch cultivation of recombinant Chinese hamster ovary (CHO) cell lines is one of the most widely used production modes for commercial manufacturing of recombinant protein therapeutics.
The technological advances in protein engineering and manufacturing of recombinant proteins have resulted in the development of a wide range of innovative biopharmaceuticals over the past three decades.
This study thus illustrates the importance of expression vector optimization for the generation of high producing stable cell lines that are essential for the manufacturing of recombinant protein therapeutics.
In this paper, we review the recent progress in the methodology of agroinfiltration, a solution to overcome the challenge of transgene delivery into plant cells for large-scale manufacturing of recombinant proteins.
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The O-xylosylation reported has implications for the manufacturing consistency of recombinant proteins containing GS linkers.
Manufacturing of stable recombinant Ig molecules is well established in a number of systems, and a large panel of reagents for detection of Ig exists.
For instance, Hyderabad-based firms Bharat Biotech and Shantha Biotechnics (now part of Paris-based Sanofi-Aventis) started out in vaccine manufacturing, and have since expanded their range of activities to include manufacturing of other recombinant products.
This work shows that expression of fusion proteins based on flagellin and M2e in plants is feasible and could became a promising approach for manufacturing of "universal" recombinant vaccines against influenza.
Manufacturing large quantities of recombinant RNAs by overexpression in a bacterial host is hampered by their instability in intracellular environment.
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