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Aeration rate is normally based on previous experiences and manufactures' recommendations.
QMC-beads were prepared according to the manufactures recommendations using 0.5, 1, 2 and 5 nmol amino modified RC24 per 1 mg beads (Dynabeads MyOne carboxylic acid, Invitrogen).
If not said otherwise, all procedures followed manufactures recommendations.
Its use followed the manufactures recommendations of 5 full clockwise rotations.
RNA was isolated for individual animals using the RNAeasy Mini kit (Qiagen) according to the manufactures recommendations.
CCL11 was measured using a Cytometric Bead Array (BD biosciences, Franklin Lakes, NJ, USA) following the manufactures recommendations.
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Pellets were either re-suspended in 20 µl complete medium and analyzed using 2-stepp cyclecycle analysis" according to manufactures recommendation by using Nucleo-Counter NC-300 (Chemometec) or re-suspended in 1 ml ice cold 70% EtOH and stored in −20 °C (1 hours).
U2932 cells were transfected with lipid-based siPORT™ NeoFX™ Transfection Agent (Ambion) according to manufactures recommendation.
All experimental protocols were based on manufactures' recommendation using the TaqMan® Universal Master Mix II (P/N 4440039).
The CD44s shRNA targeting construct was 5′-CAAGTGGACTCAACGGAGA-3′. MHCC97-H cells were transfected with either scrambled shRNA, c-Met shRNA, or CD44s shRNA using Fugene 6 transfection reagents per manufactures recommendation (Promega, Sunnyvale, CA).
Automated analyzers should be cleaned and disinfected according to manufacture recommendations or with sodium hypochlorite at a concentration of 500 ppm (1 100 dilution) (20, 21 ).
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