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Length of the product is approx.1500 bp Amplified PCR product was purified using Qiagen Mini elute Gel extraction kit according to the manufactures protocol.
Total RNA was isolated according to the manufactures protocol (Invitrogen).
Each cDNA was run on both plate A and plate B according to the manufactures protocol.
Annexin V staining was performed according to the manufactures protocol (BD Biosciences, San Jose, CA).
Mature miRNA qPCR detection was performed according to the manufactures' protocol.
according to the manufactures' protocol for real-time polymerase chain reaction (PCR) detection.
The Strep purification was done using the Strep-tag® Protein Purification Buffer Set and according to manufactures protocol (IBA).
All transfections were carried out using Lipofectamine™ 2000 Transfection Reagent (11668-019, Invitrogen) according to the manufactures protocol.
Total RNA was labeled and hybridized to Illumina HG6 v3 beadarrays (Illumina, San Diego, CA) according to manufactures protocol.
RNA was labeled according to manufactures protocol using miRCURY LNA™ microRNA Hy3/Hy5 Power labeling kit (Exiqon, Hørsholm, Denmark).
One microgram of total RNA was subjected to reverse transcription with the SYBR PrimeScriptTM RT-PCR kit (DRR063S,Takara, Dalian China) following the manufactures protocol.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com