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Cells were then magnetically sorted with streptavidin micro-beads and LS columns as per manufactures instructions (Miltenyi Biotec).
Amplicons are sequenced using the duel indexed paired end approach on the illumina MiSeq platform according to the manufactures instructions.
All enzymes and kit reagents were used according to the manufactures' instructions.
Q-PCR reactions were performed according to manufactures instructions.
PCR reactions were performed following the manufactures instructions.
SP-A was labeled with Alexa Fluor 488 (Invitrogen, Carlsbad, CA) per the manufactures instructions.
IFN-γ Secretion Assay (Miltenyi Biotech, Bergisch Gladbach, Germany) was performed according to the manufactures instructions.
Proteins were separated by SDS-PAGE and silver stained using SilverQuest (Invitrogen) following manufactures instructions.
Silver stained bands were excised, de-stained (following manufactures instructions) and speed vacuum-dried until tacky.
Two-Hybrid experiments were performed essentially as described in the manufactures instructions.
Relative levels of gene expression (fold differences) were calculated according to manufactures instructions.
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