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E.Z.N.A.™ Plasmid Mini Kit I (Omega BInc.ek, Inc., USA) was applied for plasmid extraction according to the manufactures' instruction.
DNA fragments and PCR products were excised from a 0.8% agarose gel and purified by E.Z.N.A.™ Gel Extraction Kit (200) (Omega BInc.ek, Inc., USA) according to the manufactures' instruction.
These samples were separated by Mn2+-Phos-tag SDS-PAGE [75] and detected by immunoblotting with the anti-dysbindin-1 antibody according to manufactures' instruction.
All assays were conducted following the manufactures' instruction.
The assays were performed according to manufactures' instruction.
The assay was performed according to the manufactures instruction (Enzo Life Sciences, Farmingdale, NY, USA).
Similar(39)
All enzymes and kit reagents were used according to the manufactures' instructions.
Q-PCR reactions were performed according to manufactures instructions.
PCR reactions were performed following the manufactures instructions.
SP-A was labeled with Alexa Fluor 488 (Invitrogen, Carlsbad, CA) per the manufactures instructions.
IFN-γ Secretion Assay (Miltenyi Biotech, Bergisch Gladbach, Germany) was performed according to the manufactures instructions.
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