Plasmids were electroporated using the Neon Transfection System (Invitrogen) following the manufacturers' protocol.
RiboGreen was added to solution aliquots following the manufacturers' protocol.
Total cellular RNA was isolated using Promega RNeasy Miniprep Kit (Promega, USA) following manufacturers protocol.
Subsequently, nested PCR products were purified using QIAquick purification kit (Qiagen, Hilden, Germany) following manufacturers protocol.
Apoptotic DNA fragmentation was detected by a TUNEL assay according to manufacturers' protocol.
"Instruments from different manufacturers, protocol differences, these are barriers to collaboration.
ChIP was performed according to manufacturers protocol (EZ ChIP, Upsate).
Analyses were performed according to the manufacturers protocol.
with random hexamers as per the manufacturers' protocol.
and further processing according to the manufacturers protocol.
Sequencing samples were prepared according to the manufacturers protocol (Illumina).
