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In terms of device specification, the event detection capability of a sensor is mainly dependent on its manufacture specification.
The commercial cellulase preparation Celluclast® 1.5 L from T. reesei ATCC26621 (Novozyme, Denmark) with an activity of 700 EGU/g according to manufacture specification was used in the hydrolysis experiments.
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Secondly, sensors used to detect traffic events on a vehicle may have different levels of detection capabilities, which are dependent on corresponding manufacture specifications.
This conversion rate (83.3%) is within the expected SNPlex™ performance according to manufactures specification (≥ 80%; Product Bulletin "SNPlex™ Genotyping System", [ 51]).
Guidance is offered on how to select operating parameters for optimum performance, and how to understand and compare manufactures' specifications.
The 1st strand cDNA was synthesized from 1 μg of total RNA using Superscript II Reverse Transcriptase (Invitrogen) according to manufactures specifications.
Therefore, it is proper to test the specifications of each newly acquired piece of equipment to verify it is in accordance with the manufacture's specification.
IL-12p70, IL-15 and IFN-γ in supernatants were measured by ELISA, according to manufacture's specification (R&D Systems and Biosource International).
The 5'-Full RACE kit (TaKaRa, JAPAN) was used for RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE) assay according to the manufacture's specification.
Protein content was measured by the Bio-Rad protein assay kit according to the manufacture's specification, using bovine serum albumin as standard.
After four washes in PBST, blots were treated with ECL-plus (Amersham Pharmacia) according to the manufacture's specification, and exposed to Biomax ML film (Kodak) to detect the protein bands.
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