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For D211N mutant Ty1 RT, reactions were carried out manually under the same conditions.
During the conventional CT-guided RFA procedure, insertion and placement of the RFA needle are performed manually under the guidance of real-time CT fluoroscopy.
Alcian Blue+ PanIN lesions were counted manually under the microscope, and PanIN burden calculated as the total number of Alcian Blue+ lesions per cm surface area.
Formalin-fixed paraffin-embedded primary tumor and metastatic tissue specimens were chosen for each patient and all specimens were microdissected manually under the supervision of experienced gastrointestinal pathologists.
Data captured using tape records was translated word by word into English language and color coded, organized and summarized manually under the main thematic area and presented the result by extracted concepts from main themes.
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The grade of melanophore pigment aggregation or dispersal was scored manually under light microscopy by the use of the Melanophore Index, MI (Hogben and Slome, 1931) where fully aggregated pigment give MI = 1 and fully dispersed pigment give MI = 5.
This cell was manually separated under the microscope.
Oocytes before and after PBE were manually removed under the microscope.
Whole filters were manually counted under the inverted microscope.
Cells were harvested and then cell numbers were manually counted under the microscope.
This pathogen can kill lodgepole pine (Pinus contorta) when manually inoculated under the bark at a high enough concentration [ 3, 4].
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