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The alignments were examined and manually trimmed to ensure alignment quality.
The dendrogram was manually trimmed to represent the overall similarity among diseases and identified 18 clusters.
Sequence data were manually trimmed to preserve the regions to be analyzed.
Multiple sequence alignments of these two genes were manually trimmed to the same length and concatenated.
The final alignment contained 97 taxa and was manually trimmed to the length of P. caudatum Hsp70s, resulting in an overall length of 489 amino acids including gaps.
The alignments were obtained using MAFFT with the L-INS-i option (Katoh and Standley 2013), and these alignments were manually trimmed to avoid ambiguous regions.
Similar(47)
Next, all homolog protein sequences were manually trimmed at established landmarks to create four different sequence fragments that were then used to generate HMM profiles using HMMER v. 3.1 (named TM1-4, TM6-5, TM6 and TM6-7 in Table 1, Additional file 2: Table S2 and Additional file 3: Table S3 and Figure 10) [ 34].
UGT1A1 and UGT1A6 sequences were aligned by Clustal X, adjusted manually, and trimmed to remove overhangs.
Thereafter alignments were manually inspected and trimmed to 7TM regions using Jalview software.
Alignments for internal genes were created individually using BioEdit v 7.1.11, manually inspected, and trimmed to include coding regions only.
Sequences from 3 genes were manually aligned and trimmed to include only the regions available for all isolates (VACV-COP A56R 161210–162095, E3L 51465–50929, B19R 178242–179173).
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CEO of Professional Science Editing for Scientists @ prosciediting.com