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The wastewater is slowly released into newly cleared reed beds for filtration, while the sludge from the primary tanks is periodically manually transferred to biodigesters that produce fertilizers and biogas.
When manually transferred to feeder cells, colonies with hESC-like morphology were formed (Figure 1A (d)).
Contractile CSs were manually transferred to 0.1% gelatin-coated plates for 24 hours and then dispersed into single cells by incubating in 0.25% Trypsin-EDTA (Gibco) for 30 minutes at 37°C.
The CL algorithms calculated the appropriate insulin doses, which were then automatically or manually transferred to the patch pump.
The PAO squares containing microcolonies were manually transferred to 0; 0.5; 1; 2; 4; 5; 8 and 16 μg/ml EMB.
The formed cell aggregates were manually transferred to a layer of polymerized Matrigel™ (growth factor-reduced, BD) mixed 1 1 with serum-free DMEM prior to use.
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Individual drug resistant clones were picked manually and transferred to 96-well microtiter plates, expanded and split.
Colonies were manually harvested, transferred to MEFs, and expanded according to established protocols [7], [27] Estimates of reprogramming efficiency were obtained by dividing total number of putative iPSC colonies by the input number of transduced cells.
Proteins were localised, excised manually, and transferred to sterile tubes.
The plates were incubated overnight, and the recombinants were picked manually and transferred to 96-well plates that were incubated for 10 h with shaking at 220 rpm.
The soil and CR were manually blended and transferred to close-end pots without drainage.
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