Sentence examples for manually transferred into from inspiring English sources

The individual recombinant plaques from the primary cDNA library were manually transferred into 96-well format flat bottom storage plates containing 200 ml of SM buffer (100 mM NaCl, 8 mM MgSO4, 7H2O, 50 mMTris-HCl pH 7.0, 0.04% gelatin) supplemented with 5% DMSO and 0.5% chloroform and stored temporarily at 48°C until verification of the size of cDNA insert by PCR.

Thereafter, the data were manually transferred into Excel and processed.

The paper-based forms received from the patients were manually transferred into the CIS forms by medical and administrative staff respectively.

The preparation was mixed using MultiTubevortexer (VWR Scientific Products, USA) for 20 min and the solvent-sample mixture was centrifuged at 2000 g during 10 min. The supernatant was manually transferred into a tube.

Contractile CSs were manually transferred to 0.1% gelatin-coated plates for 24 hours and then dispersed into single cells by incubating in 0.25% Trypsin-EDTA (Gibco) for 30 minutes at 37°C.

Briefly, bands were excised manually and transferred into sterile micro centrifuge tubes.

Spots of interest were excised manually and transferred into sterile microcentrifuge tubes.

All marked boxes were counted manually and transferred into a database.

Intact in vitro cultivated metacestode vesicles (H95) were manually picked, transferred into Falcon tubes and incubated in (D MEM (0.2% FCS) in the presence or absence of 100 nM insulin (saturating concentration to ensure maximal activation [ 25- 30]).

When manually transferred to feeder cells, colonies with hESC-like morphology were formed (Figure 1A (d)).

This could be used by schools accepting school fees via mobile money or small business owners and even big companies who previously needed to manually transfer their mobile payments into their bank accounts.