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All Nissl-stained neurons in the ventral horn that had a distinct nucleus were manually traced to determine area.
Van de Pol et al. [3] compared manually traced to automatically segmented hippocampi (using fluid registration) in 18 participants with Mild Cognitive Impairment assessed at two time points two years apart.
The atlas was digitized and manually traced to create the Talairach Daemon [14], an online tool that allows researchers to query for labels at five different "levels" at any given point in Talairach space.
Microtubule filaments have been manually traced to highlight arrangement.
The lung contours were manually traced to define the region of interest.
From the soma, each of the other dendrites was manually traced to its termination and associated mossy fiber synapses were identified.
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The LV endocardial border was manually traced at end systole and the region of interest was adjusted to include the entire myocardium.
Lumen and stent were manually traced at every 0.5-mm interval in stented segments.
The outer walls of individual alveoli were manually traced at both end inspiration and end expiration.
In addition, the hippocampal tail was manually traced according to the protocol described in detail in Maller et al. [1].
Annotators identified mCitrine-positive, claw-footed structures completely embedded within a tdTomato-positive ECN rosette and manually traced back to the granule cell somata.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com