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Recently, Segal et al [32] manually traced the anterior and posterior cingulate gyri, divided the anterior cingulate gyrus axially into six equal segments, and the posterior cingulate gyrus into two segments in their quest to understand the regional differences within the cingulate gyrus.
An expert rater board-certified in neurology and neurophysiology who was blinded to the intracranial EEG data reviewed the clinical MRI report and manually traced the outer regions of the visible lesions on the morphometric T1-weighted 3D volume scan based on the lesional areas identified in the initial report.
A reader manually traced the abdominal muscular wall separating the two layers.
First, we manually traced the boundary of each cell in the BF image to determine the region of individual cells.
We manually traced the shapes of single cells and measured the area of each using ImageJ (NIH).
On each of these slices the investigator manually traced the bone-cartilage boundary using predefined segmentation rules.
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Velocity-time integral (VTI) was measured by manually tracing the flow envelope for each image (Fig. 1).
Manually tracing the lesions with the internal measuring function of the magnetic resonance imaging scanner and multiplying the area by slice thickness obtained volume size.
Myocardial SI was measured after manually tracing the endocardial and epicardial contours.
Each region was determined by manually tracing the shape of BF images in the same field of view.
MVO was quantified by manually tracing the central hypoenhanced area and expressed as a percentage of LV mass.
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