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The alignment was then inspected for structural mutations and adjusted manually to preserve tandem repeat boundaries and identify inversions.
The alignment was then inspected for structural mutations and adjusted manually to preserve tandem repeat boundaries and to identify inversions.
The sequences used for comparison were obtained from the GenBank database, aligned, and then corrected manually to preserve codon alignment and conserved motifs.
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Sequence data were manually trimmed to preserve the regions to be analyzed.
Translated amino acid sequences were aligned using ClustalX [ 39], and then manually adjusted to preserve the exon correspondence among species.
The pkinase alignment was manually adjusted to preserve the conserved subdomains defined by Hanks and colleagues [ 118- 120] for eukaryotic kinases using the Se-Al Sequence Alignment Editor [ 137].
Starting from the BLAST alignment between P. brasiliensis TRR1 and KRE2 proteins with the PDB templates as found above, we manually modified them in order to preserve the secondary structures and the correspondence between cysteine residues (Additional file 6a and 6b).
Resection of the bone was performed manually using an osteotome and Kerrison rongeur to preserve local bone.
At this stage, we thought it best to preserve a smooth workflow by manually selecting only the tip electrode visible in each of the diastole images.
Vesper's technology enables voice-activated battery-powered devices to be used hands-free for long periods of time; in contrast, today's devices typically must be manually woken up with the push of a button to preserve battery life.
In order to preserve data regarding morphology and position, cells can be cut from stained tissue sections by micro-dissection, either manually or by laser assisted micro-dissection.
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