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For the ex vivo scans, volumes of interest (VOIs, ~1 cm3) were drawn manually to include aorta and carotids.
In those analyses, they scanned the available biological literatures to obtain information about associations of individual proteins with drug resistance and verified manually to include in the drug resistance list.
The angle map area was calculated from an ROI drawn manually to include V1 and V2 and then superimposed on the respective polar map.
For each single condition map (0°/blank stimulus [bs], 45°/bs, 90°/bs and 135°/bs), the pixel distribution was obtained from an ROI drawn manually to include V1 and V2.
Briefly, the 8 bit images were clipped at ±3SD from the median and color histograms were made using Image J software (Color Histogram plug-in, Kai Uwe Barthel, Internationale Medieninformatik, Berlin, Germany, http://rsbweb.nih.gov/ij/plugins/color-inspector.html) in an ROI drawn manually to include V1 and V2.
The myocardial ROI was drawn manually to include all visible ventricular activity.
Similar(51)
Regions of interest were manually placed to include the entire cell pellet area.
Thus, the boundaries of these loci were manually adjusted to include only chemotype-specific genes.
Regions of interest (ROIs) were manually defined to include the entire brain and meninges.
A consensus mtDNA sequence was extracted with samtools and bcftools [ 44], and manually edited to include also insertions and deletions.
The search results were then manually screened to include literature on the newly described HRV-C species or related strains.
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