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Briefly, 8 copies of identical amino acid sequence were manually synthesized on the core using solid phase peptide synthesis F-moc chemistry.
BP100 derived peptides were manually synthesized by the solid-phase method using Fmoc-type chemistry as previously described [ 47].
The (RQIKIWFQNRRW) peptide (Pen) with protected side chains was manually synthesized by solid phase synthesis on 2-chlorotrityl chloride resin (0.84 mmol/g).
All molecules were manually synthesized by tert-butyloxycarbonyl (t-Boc -based solid-phase pept-Boc -basedis (SPPS), following a prot-Boc -basedreported by Merrifield (1963) and later modified for multiple peptide synthesis (Houghten 1985).
In this study, we manually synthesized F-FPAMT with high yielding and radiochemical purity, and we used the customized automated synthesizer for the proof of concept of automated manufacturing of F-FPAMT.
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The PM24 parameter is designed to be determined manually, by synthesizing backward air trajectory and regional air quality information to compute 24-h back-trajectory concentrations.
Peptides were synthesized manually on solid TCP support using standard Fmoc strategy and an Intelli-Mixer syringe shaker (Neolab, Heidelberg, Germany).
Each segment of the dimeric peptides was synthesized manually on a methylbenzhydrylamine (MBHA) resin using the solid phase approach and the Boc- strategy.
The bombesin analog RM2 (BBN) [12] was synthesized manually according to standard Fmoc chemistry [13] and modified on the N-terminus with the unnatural amino acid (S -2-Amino-4-pentynoic acid with a yield of 71 % (S -2-Amino-4-pentynoic S -2-Amino-4-pentynoic).
In the preclinical setting, radiopharmaceuticals are typically synthesized manually.
F-FPTP was synthesized manually with a two-step reaction.
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