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The FGD data was transcribed manually, subjected to thematic analysis and summarized by the Moderator.
We manually subjected the top 25 PepNovo-identified hexamer tags to a BLAST search (Altschul et al, 1990) and found that 19 of these hexamer tags were consistent with their mapping to autocatalytic chymotryptic peptides (Supporting Information Table S1).
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Spots of interest were excised manually and subjected to mass spectrometric protein identification.
Spots were excised manually and subjected to in-gel digestion.
Positive yeast colonies were selected manually and subjected to yeast colony PCR followed by DNA sequencing to identify the interacting preys [ 29].
Six selected spots were excised manually and subjected to trypsin digest prior to identification by electrospray ionisation mass spectrometry on an Amazon ion trap tandem mass spectrometry (MS/MS).
Apart from the commercial and historical aspects, several other characteristics make saffron an interesting biological system: the spice is derived from the stigmas of the flower, which are harvested manually and subjected to desiccation.
The input Regions of Interest (ROIs) are segmented manually and further subjected to a number of preprocessing stages.
In order to identify these polypeptides each spot was manually excised and subjected to trypsin digestion.
Single spheres and pools of spheres were manually picked and subjected to further profiling analyses.
Spots of varying relative intensity were manually excised and subjected to in-gel tryptic digestion [ 8].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com