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A 1.2 µm × 12.6 µm region of photoactivation was manually specified for each spindle prior to imaging. 5 pre-activation frames were acquired followed by a single 0.648 s pulse from a 405 nm laser to activate the GFP-α-tubulin.
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Grid search exhaustively applies every parameter in a manually specified subset to select for parameters achieving the best performance.
An alternative to the 'Adaptive Otsu Threshold' is the 'Manual Threshold' option, where a threshold for the whole image stack can be manually specified.
The study indicates that our approach reduces the number of manually specified elements by 29%, and reduces the number of manual changes to elements for several realistic change scenarios by 52%.
This number of species exceeds the number that can be practically considered in a manually specified ODE model in which one equation would be required for each reachable species.
The Additional compute time parameter is manually specified by a user at the start of the simulation.
The radiance system, therefore, offers a computer-oriented view of appearance specification, with a set of possible operations (programs) invoked using manually specified parameters.
This bond was manually specified in a second modelling round during which ten models were constructed.
The TIM2 model was manually specified within the BEAUti input file [ 42].
We used the software BIEMS (Mulder, Hoijtink, & de Leeuw, 2012) for generating an exact data set where the mean and standard deviation of reading skills scores were manually specified.
This routine also allows predefined anchors to be manually specified that will be included in the alignment produced.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com