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Its parameters were carefully chosen after running many experiments and are not manually selected as in the case of the other Communities.
These 18 transcripts were manually selected as representatives for their potential roles in cold tolerance according to their annotations.
On-the-fly alignment was used with quality control samples manually selected as the peak list for primary alignment.
We manually selected as an input set the eight miRNAs with the most predicted targets in the process 'regulation of axonogenesis' (GO 0050770).
Glandular areas were manually selected as polygons, and positive and negative staining was assigned as class 1 and 2, respectively, with different keystrokes.
For each image, the cell body layer was identified and manually selected as a ROI from which the average pixel intensity was calculated.
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We used the PMIDs retrieved by the PubMed search as the background set and the 10 manually selected PMIDs as the training set.
∼10 of these class averages were manually selected and given as input to e2initialmodel.py from the EMAN2 package (Tang et al., 2007) to generate an initial model.
We manually selected one area as region of interest (ROI), in which the CD3‐labeled T cells had infiltrated into the tumor most densely in the specimen, when we checked it in low‐power view.
The bag ROIs were manually selected on the images as spheres of 3 cm of diameter inside the bag contour, away from the boundaries of the bag in order to avoid PVE.
Well defined spots were manually selected, excised and processed as previously described [ 116].
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