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Branch points were manually scored using ImageJ/Fiji.
Raw data were visualised and the fragments manually scored using GeneMarker v1.8 (SoftGenetics, USA).
Videos were recorded in.mov format and behaviour was manually scored using JWATCHER v1.0 [ 59].
The multiple segregation data were manually scored using a Microsoft Excel spreadsheet (Microsoft Japan, Tokyo, Japan).
Gel images in TIF format were generated by LI-COR DNA analyser and manually scored using the GelBuddy program [ 30].
Pre-recorded video was manually scored using Noldus Observer XT 7.0 software (Noldus Information Technology, Wageningen, Netherlands).
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PTM sites were manually placed and scored using sequence gazer.
Alleles were aligned and scored using Genotyper 2.5 (Perkin-Elmer) and manually binned.
Films were manually scored, scores digitized using text-recognition software (ABBYY FINEREADER), and data deconvoluted and stored in the MS Access database system "BACMan".
Importantly, the relative susceptibility indices that resulted from the RNAi construct targeting Mlo were clustered at the more resistant side of the spectrum, as reported previously for manually scored TIGS experiments using the same construct [ 5].
Films were manually scored, and scores digitized using text-recognition software (ABBYY FINEREADER).
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