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All polysomnographic data were manually scored in 30 s epochs.
Microsatellite alleles were manually scored in STRAND [ 91] using the 350 ROX™ size standard (Applied Biosystems).
Populations were manually scored in at least four to five assays using 40 to 50 worms.
Sleep stages, respiratory events, arousals and desaturations were manually scored in accordance with established criteria.
The acquired data is manually scored in 30-second segments, which eventually amounts to large workloads and high costs.
Recordings were manually scored in 30-s epochs for identification of sleep stages; each epoch was scored as Wake, N1, N2, N3 or REM sleep.
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Manually scored behaviors in this experiment were attributed across the 3D reconstruction to compare automated parameters with known behavioral states (also see Table S2 in Supporting Information).
Diagrams are generated from manually scored video in 5-min bins, over a 25-min time course (n = 6 flies).
The Masimo Radical pulse-oximeter was generally effective in identifying artefact (82.5%, 66.3%, 80.1%, 93.3% and 90.0% of total manually scored artefacts in infants at 2 weeks, 3, 6, 12 and 24 months, respectively).
Wake, NREM sleep and REM sleep were manually scored off-line (SleepSign, Kissei Comtec) in 4-s epochs according to standard criteria.
Additionally, there were significant errors in 14 (28%) of manually scored paper questionnaires.
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