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The animal was placed on the platform in the middle of the maze facing an open arm, and the number of entries and time spent (with all four paws) inside each arm were manually scored for 5 minutes.
Twenty-four-hour EEGs were manually scored for single spikes.
The mice were manually scored for performance during the trial time.
Amplification products were resolved by electrophoresis in 1.0% agarose gels containing 0.36 μg/ml ethidium bromide, photographed and manually scored for the presence of the bovine-specific amplicons.
Images of ≥100 cells per chamber manually scored for nuclear localization using the blue dapi counter stain as a nuclear localiser.
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The AFLP fingerprints were manually scored and recorded for the absence or presence of fragments; bands of equal fragment size were assumed to be homologous.
Importantly, the relative susceptibility indices that resulted from the RNAi construct targeting Mlo were clustered at the more resistant side of the spectrum, as reported previously for manually scored TIGS experiments using the same construct [ 5].
Recordings were manually scored in 30-s epochs for identification of sleep stages; each epoch was scored as Wake, N1, N2, N3 or REM sleep.
Further, number of grooming (licking the fur, scratching behavior, or wiping face) and rearing activities (exploratory behavior) were also manually scored in the open field for 5 min.
Data were collected from 25 min of manually scored video (n = 6 flies for each treatment).
Flies were recorded and their grooming movements were manually scored (see Figure 4 figure supplement 3D for ethograms).
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