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Following temporal smoothing and low pass filtering of the data, all blocks were manually scored as being either usable or unusuable for analysis.
Movement responses were manually scored as described above.
Grooming movements were manually scored as previously described (Seeds et al., 2014), with the exceptions listed below.
All individuals were manually scored as either '1' or '0' corresponding to the presence or absence of AFLP bands (100 700 bp), respectively, to construct a binary matrix.
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Manually scored behavioral video was analyzed as follows.
Each PSG recording was manually scored in 30 second epochs, with each epoch scored as either wake, Stages 1, 2, slow wave, or rapid eye movement (REM) sleep.
The ApneaLink Plus records were also manually scored after they were exported as European Data Format files into the Philips G3 Sleepware software (Philips Respironics, Carlsbad, California, USA).
Recordings were manually scored in 30-s epochs for identification of sleep stages; each epoch was scored as Wake, N1, N2, N3 or REM sleep.
Amplified fragment length polymorphism profiles were manually scored with the presence of each peak recorded as '1' and the absence of a peak as '0'.
Mitotic levels of H3K79me2 were depleted as above, and mitotic defects were manually scored.
Images of ≥100 cells per chamber manually scored for nuclear localization using the blue dapi counter stain as a nuclear localiser.
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