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BLAST (EMBL, Flybase) and domain searches (SMART) were performed to identify Drosophila proteins that contain the mucin-associated SEA, vWD4, EGF and cystein-knot domains, and identified proteins were manually scanned for the presence of PTS repeats.
Instead, the accessible data were manually scanned for red flags.
Reference lists of retrieved articles were manually scanned for all relevant additional studies and review articles.
The data was manually scanned for epochs containing eye-blinks, which were deleted from further analysis.
All electrocardiogram signals will be manually scanned for ectopic or non-sinus beats, which will be deleted from the time series.
The mouse Lif 3′UTR sequence (NM_008501.2) was manually scanned for AU-rich regions according to Hao et al. and Caput et al. [ 50, 109].
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(A – H ) We manually scanned the PDB for β-propeller structures in complex with peptide ligands.
Then, we manually scanned each tree for topologies consistent with EGTs and discarded the ones that did not contain at least C. velia, red and green algal representatives, and an outgroup.
(ii) Potential for breaches and inferring information To test for potential breaches, we manually scanned each note (500) for a potential breach.
We identified six potential sites (sites 18, 21, 28 31, data in gray), and manually scanned the R1 and R2 sequences for similar potential consensus sites that differed in only one nucleotide of the core conserved consensus, which identified an additional 15 sites which served as an expected "negative" out-group (asterisks).
Reference lists of all relevant guidelines were manually scanned and, in addition, Google Scholar was searched for additional information.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com