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Amino acid sequences were aligned with ClustalX (version 1.83) [42] and manually refined with GeneDoc (version 2.6) [43].
Models were manually refined with ViTO [23] using the sequence alignment of AmVKR, AgVKR, TcVKR, SpVKR and SmVKR.
Multiple sequence alignments were carried out with ClustalX [54] and manually refined with GeneDoc [55] to insure that the anchor residue of each helix was correctly aligned.
Models were manually refined with ViTO [ 44] using the sequence alignment of the rat mGluR1 VFTM.
The selected alignments were edited and manually refined with the program ED of the MUST package [ 51].
Sequences were aligned with the Clustal-X program [ 77] and manually refined with an alignment editor program (GeneDoc v2.5.010; [ 64]).
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Annotations were manually refined by comparison with genes of more closely related species.
These n-grams were evaluated in relation to their relevance and manually refined by translation students with knowledge of the domain.
Accession numbers and genomic locations of sequences used in this study are listed in Additional file 4. Deduced amino acid sequences were aligned with ClustalW and manually refined in BioEdit [ 57].
Nucleotide sequences were aligned with Clustal W version 1.4 [39] and manually refined using MacClade v4.05 software.
Alignments were performed using ClustalX [56] with default parameter values, and manually refined in GeneDoc where necessary.
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