Sentence examples for manually refined from from inspiring English sources

Initial sequence alignments were generated in ClustalX2 [40] and manually refined from 3D alignments of available crystal structures for hsp15.5, scL30e, hmL7Ae, scSnu13p, hsGadd45g and generated models of hsSBP2_RBD and xtGadd45a.

The final phylogenetic tree was then manually refined for visualization purposes.

We reconstructed a total of 73 vent-specific MAGs manually refined and quality-controlled from 12 metagenomes from both Piccard and Von Damm (Supplementary Fig. 2; Supplementary Data 2) with a minimum completeness of 70% and maximum redundancy of 10%.

The final phylogenetic tree was inferred from manually refined Mesquite alignment of sequences using maximum likelihood implemented in PHYML using a GTR model with estimated values for the α parameter of the Γ distribution and the proportion of invariable sites.

All the alignments were edited and manually refined using the ED program from the MUST package [47].

It is noteworthy that the previous data were obtained from very similar growth conditions and were manually refined to improve the prediction.

A multiple sequence alignment of the LI818-like sequences was generated as described above, and manually refined using BioEdit, on the basis of the crystal structure of the CAB from spinach (PDB code 1RWT; [ 42]).

For phylogenetic analysis of the Cox proteins, TBLASTX searches were carried out, the corresponding sequences recovered from GenBank and a multiple alignment generated using Clustal X and manually refined as mentioned above.

The structure was automatically built starting from an excellent multiple anomalous dispersion electron density map, and was manually refined to an Rwork of 19.9% (Rfree=23.2%), with two molecules per asymmetric unit (crystal form I; Supplementary Table S2; Supplementary Figure S4A).

Each trans-axial region-of-interest (ROI) was visually reviewed and manually refined if necessary.

Alignments were manually refined using phylogenetically conserved secondary structures.