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Individual domain of previous KATP models (PDB: 5WUA and 5TWV) and CFTR in Mg-ATP bound state (PDB: 5W81) were used as the initial model, docked into cryo-EM map with best local quality of that region by chimera and manually rebuilt in Coot according to the density (Emsley et al., 2010).
The initial model was then manually rebuilt in COOT and refined with PHENIX.
The initial model was traced automatically using the program BUCCANEER and was manually rebuilt in COOT.
The model was refined using AutoBuster and manually rebuilt in COOT 0.6.2 and O 10.0.3.
The complete rRNA was then manually rebuilt in Coot to optimize the fit to density.
The structure was refined in BUSTER (v. 2.10.1) at 3.8 Å using TLS refinement strategy (Bricogne et al., 2011) and manually rebuilt in Coot (Emsley et al., 2010).
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After placing the ligand into the electron density map, the model of the complex was manually rebuilt and refined through iterative cycles in Coot and Phenix.
The model was manually rebuilt using XtalView (McRee 1999).
The initial model was manually rebuilt using COOT [44].
The structure was manually rebuilt using the O program [35] and refined with the CNS program, with or without twin refinement.
The model was manually rebuilt using COOT (27).
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