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Their phases were manually picked with clear P- and S-wave arrivals.
Tilt-pairs of untagged Drosophila ORC particles recorded at 0° and 50° nominal stage tilt were manually picked with XMIPP.
Individual white colonies were manually picked with sterilized toothpicks and placed in wells of 384-well plates containing freezing media [ 28].
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C. sinicus were manually picked up with the aid of a stereomicroscope and preserved at −80°C pending RNA extraction.
Waveforms are band-passed from 2 to 16 Hz and then aligned with manually picked P-wave arrival times.
The epicentral distance is determined by the differential travel time of S- and P-wave with manually picked direct P- and S-wave arrival time (Additional file 1: Figure S5).
We first manually picked raw particle images with e2boxer, then did contrast transfer function correction following EMAN2 document.
Colonies were manually picked up for PCR amplification with M13 and T7 universal vector primers and subsequent sequencing with poly-T primer on an ABI Prism 3,700 sequencer (Applied Biosystems).
For each library 960 individual colonies were manually picked into 96 well plates with 14% (w/v) glycerol in TB + chloramphenicol, incubated overnight at 37°C and stored at -80°C.
The diluted cell mixture was placed on the cover of a petridish, and single cells without close neighbors were manually picked using a 10 µl pipette, with each cell contained within less than 1 µl of volume.
For the hypocenter relocation, we used the manually picked P and S arrival times, with P and S arrival time differences for 27,440 and 38,883 pairs, respectively.
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