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Both P- and S-wave arrival times from 776 ordinary earthquakes and 6 LFEs listed in the Japan Meteorological Agency (JMA) catalog were manually picked from waveforms observed by dense temporary stations as well as permanent stations.
For dataset of mTRPML1 amphipols, ~4,000 particles were manually picked in advance, and processed by 2D classification using RELION1.4 (Scheres, 2012).
After selection, drug-resistant colonies were manually picked for further expansion and screening by genomic PCR and Southern blot.
Particles were first manually picked from 20 micrographs and class averaged using RELION1.3 (Scheres, 2012).
Their phases were manually picked with clear P- and S-wave arrivals.
Islets were manually picked from the suspension using a small glass pipette and a dissecting microscope.
Leaves were manually picked from stems.
Positive transformants were manually picked for sequencing.
The iPS colonies with clear boundary were manually picked after two to three weeks.
Since not all emergent colonies likely represent iPS cell colonies by the criterion of pluripotency marker expression, putative mAdiPS cell colonies were manually picked via live immunostaining for SSEA-1 (25).
The differentially expressed and phosphorylated protein spots were manually picked from the gel and placed individually into methanol-treated tubes.
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