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Allele discrimination was manually performed according to the different PCR amplification efficiencies for different alleles, which can be shown by the cycle of threshold (Ct).
The first steps of the extraction, lysis of human cells and digestion of human DNA, were manually performed according to the manufacturer's instructions.
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Protein bands were excised manually and their in-gel trypsin digestions were performed according to Gao et al. [ 26].
Protein spots were manually excised from the gel, and in-gel digestion by trypsin was performed according to Wang et al. (2009a) with some modifications.
Attenuation correction was performed according to Chang's method, (attenuation coefficient: 0.12 cm−1), after manually drawing an ellipse around the head contour [46].
Protein expression was performed according to [26].
Neuroimaging was performed according to local practices.
Immunoblotting was performed according to [41].
ELISA was performed according to standard procedures.
Northern blots were performed according to [47].
Correlations were performed according to Pearson.
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