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The alveoli were identified in the frames and manually outlined using Photoshop CS6 (Adobe Inc).
A VOI defining each organ insert was manually outlined using the Hawkeye CT data and transferred to the SPECT image.
To quantify the migration rate of U2OS and PtK2 cells, cells were manually outlined using ImageJ software.
All neurons in each field were manually outlined using Adobe Photoshop.
For chondron size, individual chondrons were manually outlined using the MCID software, which then calculated chondron area.
In brief, the tumours were manually outlined using T2 data with freehand selection tool or the polygon selection tool.
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The resulting TIFF files were analysed quantitatively using a custom ImageJ script: the gut was manually outlined as a ROI using the polygon tool, then the RGB channels were split and the red channel subtracted from the green to eliminate background (grey) signal.
Each image was then manually outlined in ImageJ (Rasband 1997 2011) using a custom macro to automatically record a set of Cartesian coordinates defining each outline.
First, the compartments were manually outlined and average fluorescence intensities were determined using in-built functions of ImageJ software (NIH, USA).
The developed framework utilizes twenty manually outlined hippocampi (atlases) [ 44], which are used both for atlas registration, and for training the statistical image appearance models.
Fluorescence intensity of APPL1 in the cell nuclei (manually outlined based on the Hoechst staining) was calculated using the Metamorph 4.6 r10 program.
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