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The perimeter of each colony or fragment was manually outlined in every picture with Photoshop CS4.
Briefly, the surface of the pia over the barrel field was manually outlined in pictures of coronal sections.
The LV epicardial borders, and the LV and RV endocardial borders were manually outlined in end-diastolic and end-systolic frames.
Each image was then manually outlined in ImageJ (Rasband 1997 2011) using a custom macro to automatically record a set of Cartesian coordinates defining each outline.
Calculation of the interobserver agreements of the absolute tumor areas that were manually outlined in round 3 yielded an OCCC of 0.811 for the board-certified pathologists subgroup and 0.756 for the residents subgroup, respectively, showing a slight advantage for the board-certified pathologists.
Similar(55)
Automated methods using atlases defined on brain templates are used as well as VOIs being manually outlined, particularly in cases with high levels of brain atrophy.
Cell area was quantified from manually outlined cells in digitized microscopic images (recorded by an Olympus Invert scope microscope; Olympus, Tokyo, Japan) of randomly chosen cell fields using the Image-Pro Plus 6.0 software (Media Cybernetics, Silver Spring, MD).
Two observers manually outlined the hippocampus in twenty datasets, following a well-described protocol [ 49].
Subthalamic nuclei were manually outlined using FSLview in individual space by three independent researchers (AA, MCK, and CMA) using the 100-μm isotropic GRE data obtained in stage 4. Dice coefficients (Dice, 1945) were calculated to assess inter-rater variation.
In stereology, SA and WSA manual, the user manually outlined a ROI that was clearly away from the cortex/white matter demarcation, so we could be confident that only neurons in the white matter were selected.
A radiation oncologist manually outlined the gross tumor volume in the nasopharynx (GTV-nx) slice-by-slice on the pretreatment contrast-enhanced CT images in the planning system according to the MRI results.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com