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Since the cerebellum almost completely drops out in EPI images, it does not contribute any signal exceeding the baseline threshold, and thus it was not necessary to manually outline the ROI with respect to its posterior border.
Volumetric techniques are currently feasible with modern three-dimensional reconstruction stations and intraoperative navigation systems, but mostly require experienced operators to manually outline the tumor volume and then perform the analysis.
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The first category is manual and semi-automatic methods, which require manually outlining the brain regions according to a protocol [126, 127] or labeling the landmarks or seed points [128, 129].
The procedure was carried out by manually outlining the particles from several dozens of low- and high-resolution TEM images.
Tumor area was determined by manually outlining the GFP area and quantification of the surface area (mm2) was calculated using computer imaging software.
The volumes of brain anatomical regions were determined by manually outlining the periphery of the ROI on the coronal T1-weighted slices using Analyze 5.0 (Brain Imaging Resource, Mayo Clinic, Rochester, MI, USA).
We determined tumor volume on T2-weighted images by manually outlining the enhancing portion of the mass, excluding congested CSF signal in ventricles, on each image by using standard "browser" software provided with the Varian Inova imaging system.
Two observers manually outlined the hippocampus in twenty datasets, following a well-described protocol [ 49].
The somatic area was calculated using Leica Confocal Software (version 2.61), by manually outlining the soma.
LV volumes and function for every patient were assessed by manually outlining the endocardial contours of the LV from the SSFP short axes sequences [ 5].
However, all the previous ultrasonography studies measured the CSA values at separate contraction conditions by manually outlining the muscle boundary, and the continuous relationship between CSA and force was not clear yet.
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