Exact(4)
Matching sequence portions plus flanking sequences were retrieved, aligned using DiAlign [ 72] and MAFFT [ 73] and the alignment was manually optimized using Se-Al.
Amino acid sequence alignments were performed using the ClustalW multiple sequence alignment program [ 65] employing the full-length amino acid sequence, and were manually optimized using the Bioedit software [ 66].
Our interaction energy parameters in melting temperature experiments are β1 = 5.1, β2 = β2 = 0.1, σ = 0.92, β1′ = 4.1 and σ′ = 0.95, which were manually optimized using only the first data set.
Amino acid sequence alignments were performed using the ClustalW multiple sequence alignment program [ 56] employing the sequence from the first NPA motif to the start of the C-terminus (when sequence data was available), and were manually optimized using the Bioedit software [ 57].
Similar(56)
Double-stranded sequences of novel sequences generated in this study were assembled with CodonCode Aligner [ 59], automatically aligned using ClustalW [ 60], and manually inspected and optimized using Se-Al v2.0a11 [ 61].
was optimized using the process of grammatical evolution.
Models were optimized using ten-fold cross-validation.
These variables were further optimized using RSM.
These parameters were optimized using oligosaccharide standards.
The multiplexed qPCR assay was optimized using Chara DNA template.
Images were further optimized using Volocity deconvolution software.
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