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An additional 192 markers with two-point LOD <8.75 were added to linkage groups manually, of which only six markers had a two-point LOD <3.0 (2.89, 2.64, 2.12, 2.10, 2.10 and 1.80).
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A total of 298 final candidate regions with an average segment length of 1 kb were manually identified, of which 97 were detected using the B1 nucleosome positioning profile, and 201 were identified using the B2 nucleosome positioning profile (Additional file 2).
The availability of powerful knowledge discovery techniques has allowed the exploration of a greater number of possible relationships that could not be evaluated manually, the consequence of which is that modelling activity now allows previously unforeseen hypotheses to be generated.
When MTrackJ was used, the bright-field channel from the live-cell imaging was used to identify cell nuclei manually, the centre of which was used as a seed point for the position of the cells.
These include cutting cleared areas so that they have irregular edges, leaving patches of trees standing, and manually planting seedlings, all of which help speed up the process of reseeding either naturally or unnaturally.
The system allows the registration of test results both manually and automatically, all of which are double-checked prior to publishing.
Both amino-acid sequences and the conserved Interpro function domains encoded by the loci were compared to those of known Arabidopsis (TAIR10 51 and rice (MSU RGAP 6.1) genes, the outputs of which were manually checked to determine the putative functions of the loci involved in the flowering pathways.
The method is daunting: Every figure needs to be manually adjusted for each frame, of which there are 24 each second, 1440 each minute.
Clustering of the RAxML bootstrap trees using PhyloSort resulted in a "unitree" set of 68 phylogenies each of which was manually inspected to verify the topology.
MeSH to VCM alignment resulted in 10,783 relations: 1,830 of which were manually performed and 8,953 were automatically inherited.
3 motifs were extracted for the RPLP1 CNE, and 2 for the RPLP2 CNE, one of which was manually split into two upon further inspection.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com