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While especially for high cell counts, the manual counting showed a deviation of up to 400 cells/mm2 (41% variation), algorithm-determined T cell numbers generally lay in between the manually observed cell numbers but with perfect reproducibility.
Pairs were manually observed for 30 min, and copulations were recorded every minute.
There are, however, regions inside the head not covered by these ROIs, which must be manually observed during the analysis.
This table shows the extent to which each computational method recapitulates the manually observed carbon rerouting through central metabolism in the diauxic shift induced in S. cerevisiae.
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Normally human experts manually observe and rate the resistance of a large variety of sugar beet plants over a period of a few months.
The first is, as you might guess, using a powerful microscope to manually observe the cells — but this also generally uses a marker, like a fluorescent dye, to make cells visible, limiting the duration and range of effects that can be watched for.
The resin embedded specimens could be handled manually and observed freely from any angle.
Each sequence was generated twice and independently from regional cancer registry data (automatically as "algorithm sequences") and from medical record (manually as "observed sequences").
This bias setting is only required to avoid any false misdetection and is tuned manually after observing the respective energy values for a large number of simulations.
Gal-file grids were manually adjusted observing both colors and Cy3/Cy5 ratios were normalized to human and murine IgG as non-specific controls.
Two sequences possessing characteristics (indels and substitutions) of different Adh1-1 outparalogs were identified as recombinants by Recombination Detection Program RDPP) [ 54], and 13 more similar putative recombinants were identified manually by observing their conflicting positions within the alignment.
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