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One hut houses the absolute instrument, the so-called D/I fluxgate theodolite, which enables the Declination and Inclination angles of the vector magnetic field to be manually measured in "absolute" terms (for a discussion of the uncertainties associated with this instrument, see Marsal and Torta, 2007).
The OFC will be manually measured in the coronal plane.
All responses were manually measured in the same manner as described above.
Images were acquired with a fluorescence microscope and labelled DNA molecules were individually manually measured in a blind manner by ImageJ software and analysed by Excel.
Using ImageJ, the images were converted to black and white images using the threshold function, and the vesicular diameter for each was manually measured, in a protocol similar to that used for quantitation of lysosomal sizes.
Medial and lateral laxity were manually measured in full extension and in 30° of flexion, and were graded as stable (laxity less than 5°), lax (5 to 10°), or as very lax (>10°).
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A blind observer manually measured time spent in each categorized behavior.
Thus, the TEM diameters evaluated using Pebbles are consistently larger than the manually measured diameters and in excellent agreement with the diameters determined by SAXS.
When all other variables are equal, this difference in step size will result in manually measured thresholds that are on average slightly larger than automatically measured thresholds.
Tumours were manually measured throughout the experiment in two dimensions, and the volume was calculated using the ellipsoid formula: volume = 1/2 * a * b2 (a = length, b = width) [ 21].
Using the density of each food item, taken from the 2005 Canadian Nutrient File (CNF) [ 10] or manually measured (weighed by volume in triplicate), the tablespoon data for each food was converted into grams consumed per day.
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