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Manually marked and automatically extracted brushstrokes have been provided by and used with the permission of the authors of [9].
In each frame, the tip of the animal's nose was manually marked, and the co-ordinates of all 360 nose-tip positions were plotted in series to form a trace of the animal's movements around the box.
In each imaged slice, PNNs were manually marked and cross-sectional area of CSPG clouds, as well as the entire cross-sectional striatal area, were manually outlined (Neurolucida), and both CSPG cloud and PNN density measurements were obtained.
For polarization angle measurements, wound limits were manually marked and the geometrical center was selected as the wound center.
The positions of outer coat vertices were manually marked and 128 × 128 × 128 pixel subtomograms were extracted from CTF-corrected tomograms at these positions.
The numbers of cells was blindly counted within 0.25 mm of subventricular zone (SVZ), ischemic border zone (IB), and ischemic zone (IZ) using NIH image software, Image J. The immunoreactive cells were manually marked and calculated with Image J.
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An additional hint that positive tumor areas are difficult to circumscribe could be found by comparing the interobserver agreements of the manually marked tumor areas and positive tumor areas: tumor areas could be more reliably delineated (OCCC was 0.807) than positive tumor areas (OCCC was 0.704).
For the IRES RNAs, the numbers mark the five largest EPM s from the set Eγ and correspond to the manually marked EPM s in Backofen and Siebert (2007).
The pixel differences between the manually marked (x, y) coordinate and recalculated (x, y) coordinate are defined as the residual error (EOS Systems 2011).
The pathologists manually marked tumor tissue (blue) and positive tumor tissue (red).
The central region was automatically detected after the reader manually marked the most medial and lateral MR images that included the femoral condyles.
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