Sentence examples for manually isolated from from inspiring English sources

Ovarian pieces were surgically removed from six Xenopus anaesthetised adult females (Xenopus laevis), purchased from NASCO (Fort Atkinson, WI, USA). 100 Xenopus stage IV (st IV, 800-μm diameter) and 50 stage VI (st VI PI, 1200-μm diameter, in prophase I of meiosis) ovarian follicles [ 11] were manually isolated from ovarian pieces of each female.

To test this in principle, almost 70 single cells were photographed and manually isolated from the west coast of Vancouver Island, and 24 COI barcodes were generated from these single cells, although images for four single-cells in this study (GQ502036, HM236194, GQ502039, HM236195, GQ501405) were very poor quality and discarded.

Follicle-enclosed oocytes at vitellogenesis, maturation and atresia were manually isolated from the rest of the ovary using watchmaker forceps.

Mature hexadecads were manually isolated from stromata and transferred onto the center of a 10-cm malt extract agar (MEA) plate.

We amplified the gene directly using a small number of cells that were manually isolated from materials collected at each site (see Methods and Materials).

Volume of the individual cartilage plates (medial and lateral femoral condyles, medial and lateral tibial plateaus and patellar) are manually isolated from the total volume by drawing disarticulation contours around the cartilage boundaries on each section.

For immunobloting, manually isolated islets from 3 month-old male mice were homogenized in 100 μl RIPA buffer and separated in SDS-PAGE gels as previously described [ 32].

For glycan microarray analysis of organs and tissues of Asplenium elliottii (root, rhizome, petiole, lamina, vascular bundles, sclerenchyma and cortex parenchyma parenchyma) we sampled base- mid and top section of petioles and manually isolated tissues from the petioles with single edge razor blades.

Cells were manually isolated or pelleted from the culture medium.

To assess the somatic status of the mutations, we extracted DNA from manually isolated peritumoral tissue fragments (only available for 8 samples).

Neural differentiation from manually isolated pluripotent UC06 colonies was achieved as previously described by Itsykson et al. Briefly, undifferentiated hESC colonies were cultured as floating aggregates for 42 days in ultra low-binding 60 mm dishes (Corning, Corning, NY, USA).