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Cells were manually isolated or pelleted from the culture medium.
NSC can be manually isolated and be propagated as monolayer cultures for many passages [10], [11].
Follicles were manually isolated using micro tweezers under a microscope equipped with a micrometric scale in the objective.
To obtain a pure population of NSCs, rosettes were manually isolated and dissociated into single cells using Accutase.
To obtain a pure population of NSCs, rosettes were manually isolated using stretched glass Pasteur pipette and placed in fresh culture dishes.
Manually isolated or sorted NSC were cultured on 20 µg/ml poly-L-ornithine and 5 µg/ml laminin (both from Sigma) (POL) coated plates.
Similar(19)
Frequent failures of tank level controller lead to transients where the operation of shutting down RD pumps requires operators to manually isolate the quenching water and to close the main steam admission valves.
Similar to previous results, we were able to manually isolate a population of NSC from EB-rosette capable of subsequent expansion and differentiation to neurons and glia [11], [33].
The second is to manually isolate the GVs of injected oocytes, fix their contents, including the injected somatic nuclei, and stain by antibodies.
NSC contaminants were generated from a failed attempt to manually isolate NSC from SFEB.
Hemp fibres were isolated manually from stems by removing xylem using a scalpel.
Related(20)
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manually established
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manually identified
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