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Nucleotide sequences for the MLSA markers were automatically aligned with T-coffee [44] and manually inspected with MacClade [45].
All modules were manually inspected with reference to literature and relevant databases (e.g. [ 30, 32]).
All predicted CDSs were manually inspected with their GenDB observations to validate inference of the auto annotation.
The gene order and conservation of clusters were manually inspected with the gene cluster alignment results from AntiSMASH website.
Sequences were processed with the Sequencing analysis v 3.7 software (Applied Biosystems) then assembled and manually inspected with a STADEN package ver 1.5.3.
The resulting matching distances were then reported in a heatmap and manually inspected with the additional support of PFAM database (http://pfam.sanger.ac.uk).sanger.ac.uk
Similar(49)
Three strains with reduced sequence qualities (MOM_217, MOM_232, MOM_859) were manually inspected again with the Sequencher 5.0 software and included into an alignment of closely related OTUs taking into account both their morphological characterization and phylogenetic placement.
In addition, we manually inspected markers with P-value greater than 0.05 and adjusted the validation status to "SOMATIC" for 16 SNVs and 4 indels (annotated with an asterisk in Supplementary Data 6A and B, due to reduced coverage in germline DNA or tumor-in-normal contamination (e.g. IFFO1.M364fs in SJHGG003_A).
To test this hypothesis, we used the program tandem repeats finder [ 35], tabulated all repeat arrays on the 11 mega base chromosomes (data not shown) and then manually inspected repeats with a size >10 bp and a copy number >10 for aligned reads.
Defining objective criteria for residue selection in flexible docking protocols has been reported to be difficult, and consistent with this literature precedent, the remaining residues were manually inspected and residues with side chain flexibility impaired by hydrogen bonds and/or hydrophobic interactions with neighbouring residues were deselected.
Sequence data were manually inspected and trimmed, with closest taxonomic match determined by comparison with known sequences in GenBank (March 2010) using the BLASTN database search method.
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