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The crude alignment was extensively manually improved according to the conserved secondary structural elements of the motor domain, which were obtained from a superposition of all crystal structures (Kollmar, unpublished data).
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Sequences were assembled with IDBA-UD (2 ) and manually improved.
The alignment was manually improved when deemed necessary.
The resulting alignments were manually improved using the software bioedit 7.0.9 [ 33].
Before determining intron loss or gain, some alignments were manually improved.
Multiple alignments were performed with Muscle 3.6 software [ 67] and were subsequently manually improved.
The resulting model was manually improved in Coot[ 52] and refined with phenix.refine (PHENIX).
Multiple alignments were performed with Clustal W [ 41] and subsequently manually improved.
Each bulb has a dedicated app that lets you change colors manually or according to predetermined settings, among other feats.
Or maybe you prefer a pillow that you can manually adjust according to personal support level preference.
The alignment was manually adjusted according to secondary structure prediction.
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