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24 27 Second, 21 anthropometric landmarks were manually identified on facial scans by one experienced examiner (figure 2), according to their respective definitions by Farkas, 28 and their x, y and z coordinates were saved for the subsequent analysis.
Glomeruli were manually identified on these digitized images, then analyzed using Metamorph software (Molecular Devices, Sunnyvale, CA) to calculate total numbers of glomeruli as well as area and length/width diameters of each glomerulus.
Background was automatically removed by the software on the basis of selected faint spots manually identified on the master gel.
The VEGA splice variants are manually identified on the basis of transcript evidence provided by cDNAs and/or ESTs.
Twentyone facial soft tissue landmarks were manually identified on each facial image (Toma et al., 2011, 2009), and the x, y and z co-ordinates were recorded.
Subsequently, regions of interest restricted to primary visual cortex excluding the voxels with electrode susceptibility artifacts were manually identified on the basis of the anatomical image.
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Transcription start sites were manually identified based on directional RNA-Seq data or based on published data and annotated accordingly within the PGDB.
27 cell-clusters were manually identified based on surface marker expression.
The glycosidase hairpin and active sites were manually identified based on information from the sequence template (3QXF).
It should be remarked that the independent search even with 1000 initial seeds only identified ∼73% of the manually identified positions on average (Table 1), and thus showed no significant improvements while the computation time was doubled.
In order to identify full-length IS elements, the gene sequences of the transposases and surrounding genomic regions were aligned and the full-length IS elements were then manually identified based on these alignments.
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